The overall day delay was then the total day difference divided by the number of panels tested. Timing of anti-HIV detection was the time difference (days) in detection of the 1st HIV positive sample of a panel between MPD HIV Blot 2.2 and Chiron RIBA HIV-1/HIV-2 SIA. He chose the ‘western’ and not ‘eastern’ direction descriptor, to make a geographical reference to the location of his lab in Seattle, on the West Coast of the United States. It is built on a technique that involves transferring, also known as blotting, proteins separated by electrophoresis from the gel to a membrane where they can be visualized specifically. The name 'western blot' alluded to the Southern blot invented in 1975 by Ed Southern, and the northern blot invented in 1977 by James Alwine. The average score for the sensitivity of an assay was the total number of positive bleeds divided by the total number of panels tested. Western blotting remains a ubiquitous technique that is used in proteomic studies to determine protein expression levels. Western Blot (WB) is a common method to detect and analyze proteins. The number of bleedings detected positive for HIV 1/2 was counted for each panel and compared between the two test kits. The detection of HIV in the seroconversion panels by MPD HIV Blot 2.2 was compared with the Chiron RIBA HIV-1/HIV-2 SIA. Includes an anti-IgG internal control bandĪnalysis of sensitivity in seroconversion panels.Provides detection of HIV-2 antibodies on the same strip.Increases detection of early infection (seroconversion panels).Contains purified HIV-1 viral lysate proteins.Advantages of HIV Blot 2.2 Western Blot Assay

Western blot test